fCAL Assays

Multiple Applications, Evolving Assays for Inflammation to Meet Your Needs

Trust Your Calprotectin Results – Robust, Standardized, Validated and Published

Which Assay is Best for You?

The BÜHLMANN Calprotectin Assay product line has a range of solutions that services all types of users, from the subject’s home test to the high throughput labs running thousands of samples. We have a variety of test formats to best suit your needs and no matter which assay you choose results are reliable, accurate, and consistent across all the board.

Fast, Flexible, Random Access

Automation solution for mid to high throughput laboratories which can be applied on most common routine chemistry analyzers.

Gold Standard

For higher throughput, batch testing laboratory use (automation available on the multiple ELISA Processing Systems).

Rapid and Quantitative

Lateral Flow Assay for Calprotectin and Therapeutic Drug Monitoring for low throughput use.

Three Easy Steps to Help Monitor IBD

IBD patient home monitoring with the IBDoc® system.


Fast, Flexible, Random Access

BÜHLMANN fCAL® turbo is a flexible turbidimetric assay for fecal calprotectin applicable on most major clinical chemistry platforms.

The technology of immunoturbidimetry implements a further milestone in fecal calprotectin quantification. It allows for automated, rapid and flexible random access use as well as being the ideal solution for high throughput applications in the routine laboratory.

The BÜHLMANN fCAL® turbo is a turbidimetric immunoassay based on the standardization of the  BÜHLMANN fCAL® ELISA, which is globally established in laboratories as the gold standard for calprotectin measurements.

Speed: results within 10 minutes
Quality: This assay is standardized against the well established BÜHLMANN fCAL® ELISA and the PETIA methodology enhances precision and reproducibility.
Flexibility: Immunoturbiometric assays can be applied to most clinical chemistry platforms, allows random access workflow which can be directly integrated into the existing laboratory infrastructure.
Range: 20 – 8000 μg/g
Based on the established BÜHLMANN standards.
Use with CALEX® Cap device: Combined with the CALEX® CAP extraction device further simplifies the fecal calprotectin diagnostic workflow thus reducing hands-on time dramatically.

Validated Applications

Siemens Advia 1650/1800
Siemens Advia 2400
Siemens Advia XPT
Roche Cobas c501/c502
Roche cobas c701/702
Roche cobas c111
Roche cobas Integra 400+
Abbott Architect (c series)
Beckman AU Series
Beckman DxC 600/800
Ortho Vitros 5600
 Mindray BS-380
 Mindray BS-200E
 Mindray BS-400
 Mindray BS-240E
 ThermoFisher Konelab30i
 ThermoFisher Indiko
 The Binding Site Optilite
 Horiba ABX Pentra 400

Is your analyzer not listed? Please contact support@buhlmannlabs.ch and we will find a solution for your analyzer and your needs.

Method Comparison

Nilsen, T. et. al. A novel turbidimetric immunoassay for fecal calprotectin optimized for routine chemistry analyzers. J Clin Lab Anal. 2016 Sep 15. PMID:27629827

Method Comparison: fCAL® ELISA vs fCAL® turbo

K. Sunde1, M. Schneider2, T. Nilsen1, Ch. Niederberger2, Th. Jermann2, E. Sunderhagen

1Gentian AS, Moss (Norway)
2BÜHLMANN Labortories AG, Schönenbuch (Switzerland)

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BÜHLMANN fCAL® ELISA is the Gold Standard in Calprotectin testing

The kit format is a microtiter plate based assay that can be performed manually or on an automated ELISA system to reduce hands-on time.

Two protocols are available with the fCAL ELISA to cover different ranges.

The ranges are 10-600µg/g or 30-1800µg/g.

All reagents, standards and controls are provided in one kit and are ready for use.
There is no requirement for reconstituting any component, apart from the wash buffer.
This minimizes any errors as well as ensuring a long shelf life.

  • Easy-to-use ELISA kit (total assay time is 75 minutes)
  • All reagents are ready to use*
  • Kit includes calibrators and controls
  • Analytical Sensitivity: <10µg/g Calprotectin in fecal sample
  • Measurable range 30-1800µg/g or 10-600µg/g**

* except wash buffer
** depending on which protocol is used

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Quantum Blue®

Rapid and Quantitative

  • BÜHLMANN’s Quantum Blue® Product line is designed with an innovative solution based on established lateral flow technology which utilizes highly specific monoclonal antibodies to capture the molecule being measured. Optical densities of test and control lines are detected by the Quantum Blue® Reader (small, dedicated reading device) and translated into a quantitative result.
  • The Quantum Blue® Calprotectin reader analyzes the signal intensity from the test and control line to give a quantitative value and is standardized with the BÜHLMANN fCAL® ELISA.

Why Use Quantum Blue?

How to Use Quantum Blue?

  • Convenient and safe stool extraction using CALEX® Cap followed by quantitative fecal calprotectin measurement with the Quantum Blue® fCAL rapid test technology

Quantum Blue® fCAL

Comparison of Quantum Blue® to BÜHLMANN fCAL® ELISA

Assaying fCAL on Quantum Blue® LF test cassette/ cartridges (three options):

Test Quantum Blue® fCAL Quantum Blue® Calprotectin High Range Quantum Blue® Calprotectin extended
Order Code LF-CAL25 LF-CHR25 LF-CALE25
# tests per kit 25x 25x 25x
Standard Range 30-300 µg/g 100-1800 µg/g 30-1000 µg/g
> top standard report as > 300 Dilute and re-run or report >1800 report as > 1000
Final Dilution *1:2500 *1:5000 *1:500
Option 1 (Quantum Blue® fCAL)


Reagents Quantity Code Comments
Test Cassette 25 cartridges B-CAL-TC vacuum-sealed in a foil bag
Extraction Buffer 1 bottle 125 mL B-CAL-EX Ready to use
RFID Chip Card 1 B-LFCAL-RCC White plastic card
Option 2 (Quantum Blue® fCAL High Range)


Reagents Quantity Code Comments
Test Cassette 25 cartridges B-CAL-TC vacuum-sealed in a foil bag
Extraction Buffer 1 bottle 125 mL B-CAL-EX Ready to use
Chase Buffer 1 bottle 100 mL B-CHR-CB Dilution buffer; ready to use
RFID Chip Card 1 B-CHR-RCC Red plastic card
Option 3 (Quantum Blue® fCAL Extended)


Reagents Quantity Code Comments
Test Cassette 25 cartridges B-LFCALUS-TC vacuum-sealed in a foil bag
Extraction Buffer 1 bottle 125 mL B-CAL-EX Ready to use
RFID Chip Card 1 piece B-CALE-RCC White plastic card
RFID Chip Card 1 piece B-CALE-RCC720 Green plastic card

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Three Easy Steps to Help Monitor IBD
  1. Process the sample with the CALEX® Valve extraction device.
  2. Apply the extracted sample to the calprotectin cassette.
  3. Use the smartphone CalApp® to read and calculate the result and send it to the gastroenterologist via the secure IBDoc® Portal.
More Regular Patient Monitoring
  • IBDoc® provides a practical way for more frequent monitoring of patients in the privacy of their own home.
  • Under the guidance of clinicians, more regular calprotectin monitoring can help to assess if patients are in prolonged IBD remission and serve as red-flag for increased calprotectin concentrations. This approach allows for a customized, patient-centric approach for IBD disease management.
  • IBDoc® is the first self testing application for fecal calprotectin in IBD subjects. Based on BÜHLMANN’s long lasting experience with calprotectin assays, IBDoc® allows IBD subjects for convenient self testing of fecal calprotectin. CALEX® Valve has been developed for simple and easy-to-handle stool extraction. The incorporated valve allows the precise application of 60 µl onto the test cassette. The CalApp® smart phone application is available for  iOS and Android transforms smartphones into a test cassette reader.
  • BÜHLMANN IBDoc® is the first CE-marked home application for measuring the fecal inflammation marker calprotectin and has been developed based on BÜHLMANN’s long lasting experience in calprotectin assays.
  • For further information, visit www.ibdoc.net or view our IBDoc® videos.

Learn more about IBDoc®


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