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Fecal Calprotectin Assays

Multiple Applications, Evolving Assays for Inflammation to Meet Your Needs

Trust Your Calprotectin Results – Robust, Standardized, Validated and Published

Which Assay is Best for You?

The BÜHLMANN Calprotectin Assay product line has a range of solutions that services all types of users, from the subject’s home test to the high throughput labs running thousands of samples. We have a variety of test formats to best suit your needs and no matter which assay you choose results are reliable, accurate, and consistent across all the board.

Fast, Flexible, Random Access

Automation solution for mid to high throughput laboratories which can be applied on most common routine chemistry analyzers.

Gold Standard

For higher throughput, batch testing laboratory use (automation available on the multiple ELISA Processing Systems).

Rapid and Quantitative

Lateral Flow Assay for Calprotectin and Therapeutic Drug Monitoring for low throughput use.

Three Easy Steps to Help Monitor IBD

IBD patient home monitoring with the IBDoc® system.


Fast, Flexible, Random Access

BÜHLMANN fCAL® turbo is a flexible turbidimetric assay for fecal calprotectin applicable on most major clinical chemistry platforms.

The technology of immunoturbidimetry implements a further milestone in fecal calprotectin quantification. It allows for automated, rapid and flexible random access use as well as being the ideal solution for high throughput applications in the routine laboratory.

The BÜHLMANN fCAL® turbo is a turbidimetric immunoassay based on the standardization of the  BÜHLMANN fCAL® ELISA, which is globally established in laboratories as the gold standard for calprotectin measurements.

Speed: results within 10 minutes
Quality: This assay is standardized against the well established BÜHLMANN fCAL® ELISA and the PETIA methodology enhances precision and reproducibility.
Flexibility: Immunoturbiometric assays can be applied to most clinical chemistry platforms, allows random access workflow which can be directly integrated into the existing laboratory infrastructure.
Range: 20 – 8000 μg/g
Based on the established BÜHLMANN standards.
Use with CALEX® Cap device: Combined with the CALEX® CAP extraction device further simplifies the fecal calprotectin diagnostic workflow thus reducing hands-on time dramatically.

Validated Applications

Abbott Architect c-series, Alinity
Beckman AU Series (AU400/640), AU480, AU680, AU5800, DxC 700AU, DxC 600/800
Mindray BS-200E, BS-240/240Pro, BS-380, BS-400
Ortho Vitros 5600
Roche cobas c501/502, c503, c701/702, c111
Siemens Advia 1650/1800, 2400, XPT, Atellica CH 930
ThermoFisher Konelab30i, Indiko/Indiko Plus
The Binding Site Optilite
Horiba ABX Pentra 400
Biosystems BA200

*validation ongoing; **validation planned

Is your analyzer not listed? Please contact support@buhlmannlabs.ch and we will find a solution for your analyzer and your needs.

Method Comparison

Nilsen, T. et. al. A novel turbidimetric immunoassay for fecal calprotectin optimized for routine chemistry analyzers. J Clin Lab Anal. 2016 Sep 15. PMID:27629827

Method Comparison: fCAL® ELISA vs fCAL® turbo

K. Sunde1, M. Schneider2, T. Nilsen1, Ch. Niederberger2, Th. Jermann2, E. Sunderhagen

1Gentian AS, Moss (Norway)
2BÜHLMANN Labortories AG, Schönenbuch (Switzerland)

Learn more about BÜHLMANN fCAL® turbo



BÜHLMANN fCAL® ELISA is the Gold Standard in calprotectin testing

The kit format is a microtiter plate based assay that can be performed manually or on an automated ELISA system to reduce hands-on time.

Two protocols are available with the fecal calprotectin ELISA to cover different ranges.

The ranges are 10-600µg/g or 30-1800µg/g.

All reagents, standards and controls are provided in one kit and are ready for use.
There is no requirement for reconstituting any component, apart from the wash buffer.
This minimizes any errors as well as ensuring a long shelf life.

  • Easy-to-use ELISA kit (total assay time is 75 minutes)
  • All reagents are ready to use*
  • Kit includes calibrators and controls
  • Analytical Sensitivity: <10µg/g calprotectin in fecal sample
  • Measurable range 30-1800µg/g or 10-600µg/g**

* except wash buffer
** depending on which protocol is used

Learn more about BÜHLMANN fCAL® ELISA


Quantum Blue®

Rapid and Quantitative

  • BÜHLMANN’s Quantum Blue® Product line is designed with an innovative solution based on established lateral flow technology which utilizes highly specific monoclonal antibodies to capture the molecule being measured. Optical densities of test and control lines are detected by the Quantum Blue® Reader (small, dedicated reading device) and translated into a quantitative result.
  • The Quantum Blue® Reader analyzes line intensities by state of the art image analysis and translates them into a quantitative result within minutes. It is standardized with the BÜHLMANN fCAL® ELISA.

Why Use Quantum Blue®?

How to Use Quantum Blue®?

  • Convenient and safe stool extraction using CALEX® Cap followed by quantitative fecal calprotectin measurement with the Quantum Blue® fCAL rapid test technology

Quantum Blue® fCAL


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Comparison of Quantum Blue® to BÜHLMANN fCAL® ELISA

Assaying fCAL on Quantum Blue® LF test cassette/ cartridges (two options):

Test Quantum Blue® fCAL Extended Quantum Blue® fCAL High Range
Order Code LF-CALE25 LF-CHR25
Time to result 12 min (approx.) 15 min (approx.)
Sample type
Feces Feces
Standard Range 30-1000 µg/g 100-1800 µg/g
<30 µg/g <100 µg/g
Lot-specific Control Set B-CALE-CONSET
Now included in the kit

Learn more about Quantum Blue®



Three Easy Steps to Help Monitor IBD
  1. Process the sample with the CALEX® Valve extraction device.
  2. Apply the extracted sample to the calprotectin cassette.
  3. Use the smartphone CalApp® to read and calculate the result and send it to the gastroenterologist via the secure IBDoc® Portal.
More Regular Patient Monitoring
  • IBDoc® provides a practical way for more frequent monitoring of patients in the privacy of their own home.
  • Under the guidance of clinicians, more regular calprotectin monitoring can help to assess if patients are in prolonged IBD remission and serve as red-flag for increased calprotectin concentrations. This approach allows for a customized, patient-centric approach for IBD disease management.
  • IBDoc® is the first self testing application for fecal calprotectin in IBD subjects. Based on BÜHLMANN’s long lasting experience with calprotectin assays, IBDoc® allows IBD subjects for convenient self testing of fecal calprotectin. CALEX® Valve has been developed for simple and easy-to-handle stool extraction. The incorporated valve allows for the application of a precise volume onto the test cassette. The CalApp® smart phone application is available for  iOS and Android and transforms smartphones into a test cassette reader.
  • For further information, visit www.ibdoc.net or view our IBDoc® videos.

Learn more about IBDoc®